Difference between revisions of "Part:BBa K1758370"

 
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BlcR, a gene of ''Agrobacterium tumefaciens'', represses the ''blcABC''-Operon which enables the bacterium to grow on gamma-butyrolactone as carbon and energy source ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083662/ Pan et al. 2013]).  
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<h2>Usage and biology</h2>
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<p> BlcR, a gene of <i>Agrobacterium tumefaciens</i>, represses the <i>blcABC</i>-operon. The latter enables the bacterium to grow on &gamma;-butyrolactone as carbon and energy source as depicted in the following figure(<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083662">Pan et al. 2013</a>). </p>
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  <a href="https://static.igem.org/mediawiki/parts/8/82/Bielefeld-CeBiTec_blc_operon_and_reactions_image_reg.png"><img src="https://static.igem.org/mediawiki/parts/8/82/Bielefeld-CeBiTec_blc_operon_and_reactions_image_reg.png" alt="catabolism of GBL in A. tumefaciens" style="width:500px"></a>
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<p style="font-size:90%"> <i>The Blc-Operon and the catabolism of GBL in A. tumefaciens. GHB: &#947;-hydroxybutyrate; GBL: &#947;-butyrolactone; SSA: Succinic semialdehyde; SA: Succinate. </i></p>
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<h2> Characterization – BlcR function </h2>   
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<p> We performed <a data-toggle="tooltip" title="Electrophoretic Mobility Shift Assay">EMSA-shifts</a> and verified: BlcR binds to the operator site described in <a href="#Pan2013">Pan et al. 2013</a>
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Revision as of 04:56, 19 September 2015

BlcR, repressor of blcABC operon, with promoter and RBS

Usage and biology

BlcR, a gene of Agrobacterium tumefaciens, represses the blcABC-operon. The latter enables the bacterium to grow on γ-butyrolactone as carbon and energy source as depicted in the following figure(Pan et al. 2013).

catabolism of GBL in A. tumefaciens

The Blc-Operon and the catabolism of GBL in A. tumefaciens. GHB: γ-hydroxybutyrate; GBL: γ-butyrolactone; SSA: Succinic semialdehyde; SA: Succinate.


Characterization – BlcR function

We performed EMSA-shifts and verified: BlcR binds to the operator site described in Pan et al. 2013


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 812
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 520
    Illegal NgoMIV site found at 761
  • 1000
    COMPATIBLE WITH RFC[1000]