Difference between revisions of "Part:BBa K1796015:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | whole line of nif cluster | + | whole line of nif cluster. |
− | + | subparts that encode proteins contain RBS sequences. | |
− | + | ||
===Source=== | ===Source=== |
Latest revision as of 04:07, 19 September 2015
complete line of nif cluster
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2163
Illegal EcoRI site found at 6951
Illegal PstI site found at 2689
Illegal PstI site found at 6597
Illegal PstI site found at 7295
Illegal PstI site found at 7866
Illegal PstI site found at 7937
Illegal PstI site found at 8743
Illegal PstI site found at 8983 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2163
Illegal EcoRI site found at 6951
Illegal PstI site found at 2689
Illegal PstI site found at 6597
Illegal PstI site found at 7295
Illegal PstI site found at 7866
Illegal PstI site found at 7937
Illegal PstI site found at 8743
Illegal PstI site found at 8983 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2163
Illegal EcoRI site found at 6951
Illegal BglII site found at 3655
Illegal BglII site found at 10747
Illegal BamHI site found at 10570 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2163
Illegal EcoRI site found at 6951
Illegal PstI site found at 2689
Illegal PstI site found at 6597
Illegal PstI site found at 7295
Illegal PstI site found at 7866
Illegal PstI site found at 7937
Illegal PstI site found at 8743
Illegal PstI site found at 8983 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2163
Illegal EcoRI site found at 6951
Illegal PstI site found at 2689
Illegal PstI site found at 6597
Illegal PstI site found at 7295
Illegal PstI site found at 7866
Illegal PstI site found at 7937
Illegal PstI site found at 8743
Illegal PstI site found at 8983
Illegal NgoMIV site found at 5942
Illegal NgoMIV site found at 6189
Illegal NgoMIV site found at 7624
Illegal AgeI site found at 1136
Illegal AgeI site found at 2096
Illegal AgeI site found at 2451
Illegal AgeI site found at 3680
Illegal AgeI site found at 4362
Illegal AgeI site found at 4735
Illegal AgeI site found at 5202 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2268
Design Notes
whole line of nif cluster. subparts that encode proteins contain RBS sequences.
Source
Paenibacillus sp.,promoted