Difference between revisions of "Part:BBa K1807005:Experience"
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| − | The Ap PPK UW-1 does show significant increase in phosphate uptake/storage. | + | The Ap PPK UW-1 protein generator does show significant increase in phosphate uptake/storage. |
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 03:58, 19 September 2015
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how you used this part and how it worked out.
Applications of BBa_K1807005
Figure 1. Graph showing the phosphate concentration within the cell lysate of 10^8, cells for 5 different ppk variants after 6 hours; ; Δppk Ap SK12 - Candidatus Accumulibacter phosphatis SK-12 strain ppk variant, Δppk Ap UW1- Candidatus Accumulibacter phosphatis UW-1 strain ppk variant, Δppk Ap BA91- Candidatus Accumulibacter phosphatis BA-91 strain ppk variant, Δ ppk is Escherichia coli with ppk knockout using kanamycin KEIO collection. BW25113 is the Escherichia coli strain used for the experiment. One way ANOVA was used and overall there was a statistically significant difference between groups and post-hoc LSD was used; NS signifies no statistical significance, *** signifies p<0.001. (ANOVA: F=48.503; d.f. = 14, p<0.001) Results were measured using a BMG FLUOstar Omega plate reader at OD650 and concentrations calculated from known phosphate concentration standards.
Figure 2. Graph showing uptake of phosphate after 6 hours from the media for 5 different ppk variants standardized against cell amount (1 OD600); Δppk Ac SK12 - Candidatus Accumulibacter phosphatis SK-12 strain ppk variant, Δppk Ac UW1- Candidatus Accumulibacter phosphatis UW-1 strain ppk variant, Δppk Ac BA91- Candidatus Accumulibacter phosphatis BA91 strain ppk variant, Δ ppk is Escherichia coli with ppk knockout using kanamycin KEIO collection. BW25113 is the Escherichia coli strain used for the experiment. One way ANOVA was used and the result showed overall there was statistically significant different. Post-hoc LSD was used; NS signifies no statistical significance, * signifies p<0.05. (ANOVA: F=3.810; d.f. = 14, p<0.05) Results were measured using a BMG FLUOstar Omega plate reader at OD650 and concentrations calculated from known phosphate concentration standards.
Result
The Ap PPK UW-1 protein generator does show significant increase in phosphate uptake/storage.
User Reviews
UNIQddb64169c5b83b26-partinfo-00000002-QINU UNIQddb64169c5b83b26-partinfo-00000003-QINU