Difference between revisions of "Part:BBa K1807002:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K1807002=== | ===Applications of BBa_K1807002=== | ||
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| + | <img src="https://static.igem.org/mediawiki/2015/7/76/Phosphate13_TeamYork.png" style="width:65%;height:auto"> | ||
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| + | Figure 1. Graph showing the phosphate concentration within the media for 5 different ppk variants and 1 ppx variant after 6 hours. Δppk Ecppk- native <i>Escherichia coli</i> ppk variant, Ecppx- native <i>Escherichia coli </i> ppx variant, Δ ppk is <i>Escherichia coli</i> with ppk knockout from the kanamycin insertion KEIO collection, Δppk Koppk - <i>Kingella oralis</i> ppk variant, PKDM12 <i>Escherichia coli</i> ppk variant from the Keasling collection. BW25113 is the parent <i>Escherichia coli</i> strain used in the experiment. One way ANOVA was used and overall there was no statistical difference between strains. Post-hoc LSD was also performed and there was statistical difference between Δppx and BW25113. * signifies there is p< 0.05.(ANOVA: F=1.616; d.f. = 17, p<0.229). Results were measured using a BMG FLUOstar Omega plate reader at OD<sub>650</sub> and concentrations calculated from known phosphate concentration standards. | ||
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| + | <img src="https://static.igem.org/mediawiki/2015/2/21/Phosphate12_TeamYork.png" style="width:65%;height:auto"> | ||
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| + | Figure 2. Graph showing the phosphate concentration of cell lysate of 10^8, cells for 5 different ppk variants and 1 ppx variant after 6 hours. ; Δppk Ecppk- native <i>Escherichia coli</i> ppk variant, Ecppx- native <i>Escherichia coli</i> ppx variant, Δ ppk is <i>Escherichia coli</i> with ppk knockout from the kanamycin insertion KEIO collection, Δppk Koppk - <i>Kingella oralis</i> ppk variant, pKDM12 <i>Escherichia coli</i> ppk variant from the Keasling collection. BW25113 is the parent <i>Escherichia coli</i> strain used in the experiment. No statistical difference was shown. Results were measured using a BMG FLUOstar Omega plate reader at OD<sub>650</sub> and concentrations were calculated from known phosphate concentration standards. | ||
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| + | ===Result=== | ||
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| + | The Ec PPK protein generator does not show significant increase or decrease in phosphate uptake/storage. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 03:58, 19 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1807002
Figure 1. Graph showing the phosphate concentration within the media for 5 different ppk variants and 1 ppx variant after 6 hours. Δppk Ecppk- native Escherichia coli ppk variant, Ecppx- native Escherichia coli ppx variant, Δ ppk is Escherichia coli with ppk knockout from the kanamycin insertion KEIO collection, Δppk Koppk - Kingella oralis ppk variant, PKDM12 Escherichia coli ppk variant from the Keasling collection. BW25113 is the parent Escherichia coli strain used in the experiment. One way ANOVA was used and overall there was no statistical difference between strains. Post-hoc LSD was also performed and there was statistical difference between Δppx and BW25113. * signifies there is p< 0.05.(ANOVA: F=1.616; d.f. = 17, p<0.229). Results were measured using a BMG FLUOstar Omega plate reader at OD650 and concentrations calculated from known phosphate concentration standards.
Figure 2. Graph showing the phosphate concentration of cell lysate of 10^8, cells for 5 different ppk variants and 1 ppx variant after 6 hours. ; Δppk Ecppk- native Escherichia coli ppk variant, Ecppx- native Escherichia coli ppx variant, Δ ppk is Escherichia coli with ppk knockout from the kanamycin insertion KEIO collection, Δppk Koppk - Kingella oralis ppk variant, pKDM12 Escherichia coli ppk variant from the Keasling collection. BW25113 is the parent Escherichia coli strain used in the experiment. No statistical difference was shown. Results were measured using a BMG FLUOstar Omega plate reader at OD650 and concentrations were calculated from known phosphate concentration standards.
Result
The Ec PPK protein generator does not show significant increase or decrease in phosphate uptake/storage.
User Reviews
UNIQa6c39194a94c2a41-partinfo-00000002-QINU UNIQa6c39194a94c2a41-partinfo-00000003-QINU