Difference between revisions of "Part:BBa K1603003"
 
(3 intermediate revisions by the same user not shown)
Line 2: Line 2:
 
<partinfo>BBa_K1603003 short</partinfo>
 
<partinfo>BBa_K1603003 short</partinfo>
  
−
Recombinase A from Deinococcus Radiodurans. Used in DNA-repair mechanisms.  
+
Recombinase A from ''Deinococcus radiodurans''. Used in DNA-repair mechanisms.  
−
Codon optimized for ''saccharomyces cerevisiae'' and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair
+
Improved [https://parts.igem.org/wiki/index.php/Part:BBa_K1797011 RecA] by codon optimization for ''Saccharomyces cerevisiae'' and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair
  
  

Latest revision as of 03:45, 19 September 2015

RecA - optimized for yeast

Recombinase A from Deinococcus radiodurans. Used in DNA-repair mechanisms. Improved RecA by codon optimization for Saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1116
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1032