Difference between revisions of "Part:BBa K1631000"
 
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<partinfo>BBa_K1631000 short</partinfo>
 
<partinfo>BBa_K1631000 short</partinfo>
  
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This part is improvement of BBa_I716211. We added a start codon and charactarized it. If you want to know more detail, visit our wiki.
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<p>This part is improvement of [[Part:BBa_I716211]]. We added a start codon and charactarized it. If you want to know more detail, visit our wiki.
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<br>Barnase is a RNase from <i>Bacillus amyloliquefaciens</i>. Its cytotoxity can be neutralized by immunity protein called Barstar ([[Part:BBa_K1631007]]).
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<br>This coding sequence only contains catalitic domain (not include extracellular translocation signal peptide of <i>Bacillus amyloliquefaciens</i>).</P>
  
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Barnase is a RNase from Bacillus amyloliquefaciens. Its cytotoxity can be neutralized by immunity protein called Barstar (BBa_).
 
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This coding sequence only contains catalitic domain (not include extracellular translocation signal peptide of Bacillus amyloliquefaciens).
 
  
  
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<!-- Add more about the biology of this part here
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<!-- <a href="https://parts.igem.org/Part:BBa_I716211">BBa_I716211</a>
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<!-- Add more about the biology of this part here -->
 
===Usage and Biology===
 
===Usage and Biology===
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We succeeded to control growth rate of <i>E coli</i>.
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<p>We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control.
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We measured OD600 of 3 samples for each condition.
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[[File:Yamanaka 002.png|600px|thumb|center|alt text]]
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</p>
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We also evaluated the growth rate by slope of next graph.
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[[File:Yamanaka 001.png|600px|thumb|center|alt text]]
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<br>Plac GFP-          0.0213(/min)
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<br>Plac GFP+          0.0205(/min)
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<br>Plac Barnase GFP-  0.0222(/min)
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<br>Plac Barnase GFP+  0.0117(/min)
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Latest revision as of 03:43, 19 September 2015

Translational unit of Barnase

This part is improvement of Part:BBa_I716211. We added a start codon and charactarized it. If you want to know more detail, visit our wiki.
Barnase is a RNase from Bacillus amyloliquefaciens. Its cytotoxity can be neutralized by immunity protein called Barstar (Part:BBa_K1631007).
This coding sequence only contains catalitic domain (not include extracellular translocation signal peptide of Bacillus amyloliquefaciens).


Usage and Biology

We succeeded to control growth rate of E coli.

We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control. We measured OD600 of 3 samples for each condition.

alt text

We also evaluated the growth rate by slope of next graph.

alt text


Plac GFP- 0.0213(/min)
Plac GFP+ 0.0205(/min)
Plac Barnase GFP- 0.0222(/min)
Plac Barnase GFP+ 0.0117(/min)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]