Difference between revisions of "Part:BBa K1679019"
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<partinfo>BBa_K1679019 short</partinfo>
 
<partinfo>BBa_K1679019 short</partinfo>
  
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This is a Translational_Unit containing,a RBS (BBa_B0034),a GFP coding sequence (BBa_E0040) ,two terminator(BBa_B0010 and BBa_B0012),a promoter(BBa_J23119K),,a RNA thermometer (BBa_K115003) and a RFP coding sequence (BBa_E0010) on the pSB1C3, which can be easily transformed into E.coli.
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This is a Translational_Unit containing, a RBS (BBa_B0034), a GFP coding sequence (BBa_E0040), two terminator(BBa_B0010 and BBa_B0012), a promoter(BBa_J23119K),a RNA thermometer (BBa_K115002) and a RFP coding sequence (BBa_E0010) on the pSB1C3, which can be easily transformed into ''E.coli''.
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There is a RFP reproter device downstream.It has a RNA Thermometers(RNAT) which are temperature-sensing RNA sequences in 5’UTR of their mRNAs. At low temperature, RNAT folds into structure, blocking access of ribosome; at high temperature, RNAT switch from off to open conformation, increasing the efficiency of translation initiation.When the temperature rises above 37°C, RFP would be expressed in theory.
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There is a GFP reporter device except promoter in front of the RFP reproter device.
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This is Translational_Unit that a GFP reporter device except promoter in front of a RFP reprorter device.
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It's a intermediate before we constructing [https://parts.igem.org/Part:BBa_K1679030 BBa_K1679030]
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1679019 SequenceAndFeatures</partinfo>

Latest revision as of 03:20, 19 September 2015

GFP+Promoter+RNA thermometer (FourU) +mRFP

This is a Translational_Unit containing, a RBS (BBa_B0034), a GFP coding sequence (BBa_E0040), two terminator(BBa_B0010 and BBa_B0012), a promoter(BBa_J23119K),a RNA thermometer (BBa_K115002) and a RFP coding sequence (BBa_E0010) on the pSB1C3, which can be easily transformed into E.coli.

This is Translational_Unit that a GFP reporter device except promoter in front of a RFP reprorter device. It's a intermediate before we constructing BBa_K1679030

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 890
    Illegal NheI site found at 913
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1539
    Illegal AgeI site found at 1651
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662