Difference between revisions of "Part:BBa K1760001"

Revision as of 03:12, 19 September 2015

TodE

Designed biobrick is a composite part that contains a TetO promoter, RBS optimized by using Salis RBS calculator and codon optimized todE gene for E.Coli. TodE gene is part of a tod operon found in Pseudomonas Putida F1 species. TodE codes for TodE expresses Catechol 2-3 dioxygenase protein. It plays a major role in degradation of toluene. Specifically, todE degrades 3-methylcatechol, a highly toxic intermediate into HOD.

In pseudomonas putida species, HOD is aerobically metabolized through TCA cycle. Theoretically, a E.Coli MG1655 type bacteria containing todC1C2BADEF genes can degrade toluene through TCA cycle by using its own native enzymes.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 997
1000
COMPATIBLE WITH RFC[1000]

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-Designed biobrick is a composite part that contains a TetO promoter, RBS optimized by using Salis RBS calculator and codon optimized todE gene for E.Coli. TodE gene is part of a tod operon found in Pseudomonas Putida F1 species. It plays a major role in degradation of toluene. Specifically, todE degrades 3-methylcatechol, a highly toxic intermediate into HOD.
+Designed biobrick is a composite part that contains a TetO promoter, RBS optimized by using Salis RBS calculator and codon optimized todE gene for E.Coli. TodE gene is part of a tod operon found in Pseudomonas Putida F1 species. TodE codes for TodE expresses Catechol 2-3 dioxygenase protein. It plays a major role in degradation of toluene. Specifically, todE degrades 3-methylcatechol, a highly toxic intermediate into HOD.
 https://static.igem.org/mediawiki/2015/1/16/Toronto_2015_Pathway.png
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 [[File:TodEonly.png]]
-TodE expresses Catechol 2-3 dioxygenase
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