Difference between revisions of "Part:BBa K1760001"
| Line 7: | Line 7: | ||
https://static.igem.org/mediawiki/2015/1/16/Toronto_2015_Pathway.png | https://static.igem.org/mediawiki/2015/1/16/Toronto_2015_Pathway.png | ||
| − | + | [[File:TodEonly.png]] | |
| + | TodEonly.png | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 03:05, 19 September 2015
TodE
TodE expresses Catechol 2-3 dioxygenase Designed biobrick is a composite part that contains a TetO promoter, RBS optimized by using Salis RBS calculator and codon optimized todE gene for E.Coli. TodE gene is part of a tod operon found in Pseudomonas Putida F1 species. It plays a major role in degradation of toluene. Specifically, todE degrades 3-methylcatechol, a highly toxic intermediate into HOD. In pseudomonas putida species, HOD is aerobically metabolized through TCA cycle. Theoretically, a E.Coli MG1655 type bacteria containing todC1C2BADEF genes can degrade toluene through TCA cycle by using its own native enzymes.
TodEonly.png
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 997
- 1000COMPATIBLE WITH RFC[1000]