Difference between revisions of "Part:BBa K1692001"
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This part includes a T7 promoter, allowing for inducible expression | This part includes a T7 promoter, allowing for inducible expression | ||
− | See our | + | See our [https://parts.igem.org/Part:BBa_K1692002 synthesized FDC] for more results. |
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Latest revision as of 02:58, 19 September 2015
Ferulic acid decarboxylase (FDC) with T7 promoter and RBS
Ferulic Acid Decarboxylase with T7 promoter
Ferulic acid decarboxylase (FDC) catalyzes the conversion of trans-cinnamic acid to styrene. We isolated this genetic part from Saccharomyces cerevisiae and inserted the protein-coding sequence into the pSB1C3 backbone. The original sequence in yeast contains an SpeI restriction site in the 999th nucleotide position. Thus, we performed site-directed mutagenesis in order to make our part BioBrick compatible. Gene sequencing analysis confirmed that our site-directed mutagenesis was successful. This part includes a T7 promoter, allowing for inducible expression See our synthesized FDC for more results.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 798