Difference between revisions of "Part:BBa K1595024:Experience"
Line 20: Line 20:
  
 
<h3>Overview of Characterization of MBP+EcnB BioBrick</h3>
 
<h3>Overview of Characterization of MBP+EcnB BioBrick</h3>
−
As described on the <a href="http://2015.igem.org/Team:Cornell/wetlab">2015 Cornell iGEM wiki</a> section, the peptide EcnB is capable of inhibiting the growth of <i>F. psychrophillum</i> in order to treat bacterial coldwater disease prevalent in salmonid species. In order to stabilize the EcnB peptide, we have placed EcnB downstream of MBP, a maltose binding protein.
+
As described on the Cornell iGEM wiki, the peptide EcnB is capable of inhibiting the growth of <i>F. psychrophillum</i> in order to treat bacterial coldwater disease prevalent in salmonid species. In order to stabilize the EcnB peptide, we have placed EcnB downstream of MBP, a maltose binding protein. We ran a series of disk diffusion assays from lysate from BBa_K1595024 as compared to antibiotics used in the field (oxytetracycline) and BL21 lysate as a negative control. <br>

Revision as of 02:53, 19 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1595024

User Reviews

UNIQ3543e44dfb9e81f1-partinfo-00000000-QINU UNIQ3543e44dfb9e81f1-partinfo-00000001-QINU

Overview of Characterization of MBP+EcnB BioBrick

As described on the Cornell iGEM wiki, the peptide EcnB is capable of inhibiting the growth of F. psychrophillum in order to treat bacterial coldwater disease prevalent in salmonid species. In order to stabilize the EcnB peptide, we have placed EcnB downstream of MBP, a maltose binding protein. We ran a series of disk diffusion assays from lysate from BBa_K1595024 as compared to antibiotics used in the field (oxytetracycline) and BL21 lysate as a negative control.