Difference between revisions of "Part:BBa K1648004"
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Different genes can be added into this part at multiple restriction sites, so that different proteins will be expressed on the magnetosome after transformation of the part into <i>A. vinelandii</i> genome via <b>random integration</b>. | Different genes can be added into this part at multiple restriction sites, so that different proteins will be expressed on the magnetosome after transformation of the part into <i>A. vinelandii</i> genome via <b>random integration</b>. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 02:48, 19 September 2015
Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane
Consist of a gene (mamC) from magnetosome island of Magnetospirillum gryphiswaldense genome, a promotor & RBS(J13002) in front of multiple restriction sites and a double terminator(B0015) at the back of multiple restriction sites.
Different genes can be added into this part at multiple restriction sites, so that different proteins will be expressed on the magnetosome after transformation of the part into A. vinelandii genome via random integration.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2759
Illegal XbaI site found at 2765
Illegal SpeI site found at 1
Illegal PstI site found at 7 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2759
Illegal SpeI site found at 1
Illegal PstI site found at 7 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2759
Illegal XhoI site found at 1162
Illegal XhoI site found at 2188 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2759
Illegal XbaI site found at 2765
Illegal SpeI site found at 1
Illegal PstI site found at 7 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2759
Illegal XbaI site found at 2765
Illegal SpeI site found at 1
Illegal PstI site found at 7
Illegal NgoMIV site found at 2546
Illegal NgoMIV site found at 2636 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 2611