Difference between revisions of "Part:BBa K1820024"

Latest revision as of 02:25, 19 September 2015

Phage inducible promoter 1997 +RBS+FspI + term

A sequence designed to provide a kill switch for Lactococcus lactis in the case of an infection by P335 species lactococcal phage Φ31.

Usage and Biology

The promoter in this sequence is A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31, containing two tandem transcription sites. Transcription from this promoter is induced after the phage genome is inserted into the bacterial cell, resulting in downstream expression in the initial stages of infection. It has been previously demonstrated that expression is successfully induced from this promoter and only occurs during phage infection. Once the promoter has been activated, the construct will produce FspI, which will kill the L. lactis cell.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 42
Illegal SapI.rc site found at 492

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 __NOTOC__
 <partinfo>BBa_K1820024 short</partinfo>
-Add
+A sequence designed to provide a kill switch for ''Lactococcus lactis'' in the case of an infection by P335 species lactococcal phage Φ31.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+The promoter in this sequence is A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31, containing two tandem transcription sites. Transcription from this promoter is induced after the phage genome is inserted into the bacterial cell, resulting in downstream expression in the initial stages of infection. It has been previously demonstrated that expression is successfully induced from this promoter and only occurs during phage infection. Once the promoter has been activated, the construct will produce FspI, which will kill the ''L. lactis'' cell.
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