Difference between revisions of "Part:BBa K1679014:Design"
m |
m (→Design Notes) |
||
| Line 6: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | We use a RNA thermometer to make the device temperature-sensitive. When the temperature is below 37°C,the secondary structure of the RBS after transform would be folded, so that the ribosome can't translated RFP. When the temperature rises above | + | We use a RNA thermometer to make the device temperature-sensitive. When the temperature is below 37°C,the secondary structure of the RBS after transform would be folded, so that the ribosome can't translated RFP. When the temperature rises above 37°C, the secondary structure of the RBS after transform would be unfolded and produce RFP. |
We designed this part for measurement of the switch activity of K115002. | We designed this part for measurement of the switch activity of K115002. | ||
| − | |||
| − | |||
| − | |||
===Source=== | ===Source=== | ||
Latest revision as of 02:24, 19 September 2015
RNA thermometer (FourU) +mRFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 613
Illegal AgeI site found at 725 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We use a RNA thermometer to make the device temperature-sensitive. When the temperature is below 37°C,the secondary structure of the RBS after transform would be folded, so that the ribosome can't translated RFP. When the temperature rises above 37°C, the secondary structure of the RBS after transform would be unfolded and produce RFP. We designed this part for measurement of the switch activity of K115002.
Source
BBa_K115002 BBa_E1010
References
Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches[J]. Nature Reviews Microbiology, 2012, 10(4):255-265.