Difference between revisions of "Part:BBa K1679015:Design"

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===Design Notes===
 
===Design Notes===
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We use a RNA thermometer to make the device temperature-sensitive. When the temperature is below 37°C,the secondary structure of the RBS after transform would be folded, so that the ribosome can't translated RFP. When the temperature rises above 42°C, the secondary structure of the RBS after transform would be unfolded and produce RFP.  
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We use a RNA thermometer to make the device temperature-sensitive. When the temperature is below 37°C,the secondary structure of the RBS after transform would be folded, so that the ribosome can't translated RFP. When the temperature rises above 37°C, the secondary structure of the RBS after transform would be unfolded and produce RFP.  
 
We designed this part for measurement of the switch activity of K115003.
 
We designed this part for measurement of the switch activity of K115003.
  

Latest revision as of 02:13, 19 September 2015

RNA thermometer (PrfA) +mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 670
    Illegal AgeI site found at 782
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use a RNA thermometer to make the device temperature-sensitive. When the temperature is below 37°C,the secondary structure of the RBS after transform would be folded, so that the ribosome can't translated RFP. When the temperature rises above 37°C, the secondary structure of the RBS after transform would be unfolded and produce RFP. We designed this part for measurement of the switch activity of K115003.



Source

BBa_K115003 BBa_E1010

References

Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches[J]. Nature Reviews Microbiology, 2012, 10(4):255-265.