Difference between revisions of "Part:BBa K1789008:Design"
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===Source=== | ===Source=== | ||
| − | This part is built by NJU_CHINA using | + | This part is built by NJU_CHINA using Golden Gate technique. We standardized this part by ourselves. |
===References=== | ===References=== | ||
Latest revision as of 02:00, 19 September 2015
TALE1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2082
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1075
Design Notes
This part is designed by ourselves based on the sequence of BM1 in our project.
NJU_CHINA finished the primary construction of this part and edited the vectors provided by the Golden Gate TALEN and TAL Effector Kit 2.0 (adding corresponding cutting sites to be specific) to meet the RFC10 bio-brick standard. We then finished the standardization of this part b ourselves.
Fig. 1 The Sequence of TALE1 cDNA and deduced amino acids
The protocol can be reached in our wiki's protocol page.
Source
This part is built by NJU_CHINA using Golden Gate technique. We standardized this part by ourselves.