Difference between revisions of "Part:BBa K1789008:Design"

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===Design Notes===
 
===Design Notes===
  
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This part is designed based on the sequence of BM1 in our project.
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This part is designed by ourselves based on the sequence of BM1 in our project.
  
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NJU_CHINA finished the primary construction of this part and edited the vectors provided by the Golden Gate TALEN and TAL Effector Kit 2.0 (adding corresponding cutting sites to be specific) to meet the RFC10 bio-brick standard.
+
NJU_CHINA finished the primary construction of this part and edited the vectors provided by the Golden Gate TALEN and TAL Effector Kit 2.0 (adding corresponding cutting sites to be specific) to meet the RFC10 bio-brick standard. We then finished the standardization of this part b ourselves.
  
 
[[File:TALE1.jpg|800px|]]
 
[[File:TALE1.jpg|800px|]]
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The protocol can be reached in our wiki
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The protocol can be reached in our wiki's protocol page.
  
 
===Source===
 
===Source===
  
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This part is built by NJU_CHINA using GATEWAY technique. We standardized this part by ourselves.  
+
This part is built by NJU_CHINA using Golden Gate technique. We standardized this part by ourselves.
  
 
===References===
 
===References===

Latest revision as of 02:00, 19 September 2015

TALE1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2082
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1075


Design Notes

This part is designed by ourselves based on the sequence of BM1 in our project.

NJU_CHINA finished the primary construction of this part and edited the vectors provided by the Golden Gate TALEN and TAL Effector Kit 2.0 (adding corresponding cutting sites to be specific) to meet the RFC10 bio-brick standard. We then finished the standardization of this part b ourselves.

TALE1.jpg

Fig. 1 The Sequence of TALE1 cDNA and deduced amino acids


The protocol can be reached in our wiki's protocol page.

Source

This part is built by NJU_CHINA using Golden Gate technique. We standardized this part by ourselves.

References