Difference between revisions of "Part:BBa K1679005"

Revision as of 01:49, 19 September 2015 (view source) QinQikai (Talk | contribs) (→‎Plate Reader Result) ← Older edit		Revision as of 01:53, 19 September 2015 (view source) QinQikai (Talk | contribs) (→‎Plate Reader Result) Newer edit →
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	TR: Technical Replicate<BR>BR: Biological Replicate</p>		TR: Technical Replicate<BR>BR: Biological Replicate</p>
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Revision as of 01:53, 19 September 2015

GFP Reporter

This is a device that contain promoter BBa_J23117, RiboJ and BBa_I13504.

Plate Reader Result

Equipment

Varioskan Flash Multimode Reader
Thermo Scientific
Read Speed: 6.4 well per second

Software

Run Software Version: Skanlt Software 2.4.5 RE for Varioskan Flash
Current Software Version: Skanlt Software 2.4.5 RE for Varioskan Flash

Protocol

Date: 2015 Aug 12
1. Set our instrument to read OD600
2. Setup a 96-well plate with our cultures
3. Take the measurement and record it
4. Calculate the dilution required for each sample (OD600=0.5)
5. Dilute each sample
6. Remeasure our sample on OD600
7. Recalculate our dilution and remeasure until it's within 5% of 0.5.

Unit

Three technical replicates of M9 liquid media was measured, which were called background value.
A series of concentration of sodium fluorescein was measured, which are 0, 10, 20, 40, 80, 160, 320, 640 ng/mL . A calibration curve was defined.

Dataset

All samples were cut the mean of background value, and then compared with the calibration curve to get the final dataset in units of fluorescein.