Difference between revisions of "Part:BBa K1648004"

Revision as of 01:51, 19 September 2015

Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane

Construct Map for K1648004

Consist of a gene (mamC) from magnetosome island of Magnetospirillum gryphiswaldense genome, a promotor & RBS(J13002) in front of multiple restriction sites and a double terminator(B0015) at the back of multiple restriction sites.

Different genes can be added into this part at multiple restriction sites, so that different proteins will be expressed on the magnetosome after transformation of the part into A. vinelandii genome via random integration.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2759
Illegal XbaI site found at 2765
Illegal SpeI site found at 1
Illegal PstI site found at 7
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2759
Illegal SpeI site found at 1
Illegal PstI site found at 7
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2759
Illegal XhoI site found at 1162
Illegal XhoI site found at 2188
23
INCOMPATIBLE WITH RFC[23]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2759
Illegal XbaI site found at 2765
Illegal SpeI site found at 1
Illegal PstI site found at 7
25
INCOMPATIBLE WITH RFC[25]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2759
Illegal XbaI site found at 2765
Illegal SpeI site found at 1
Illegal PstI site found at 7
Illegal NgoMIV site found at 2546
Illegal NgoMIV site found at 2636
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 2611

@@ Line 4: / Line 4: @@
 [[File:K1648004.png|350px|thumb|right|Construct Map for K1648004]]
-Consist of a genome (mamC) from magnetosome island of ''Magnetospirillum gryphiswaldense'' genome.
+Consist of a gene (mamC) from magnetosome island of ''Magnetospirillum gryphiswaldense'' genome, a promotor & RBS([https://parts.igem.org/Part:BBa_J13002 J13002]) in front of multiple restriction sites and a double terminator([https://parts.igem.org/Part:BBa_B0015 B0015]) at the back of multiple restriction sites.
+Different genes can be added into this part at multiple restriction sites, so that different proteins will be expressed on the magnetosome after transformation of the part into <i>A. vinelandii</i> genome via <b>random integration</b>.