Difference between revisions of "Part:BBa K1585203"

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|<html> <img src="https://static.igem.org/mediawiki/parts/0/05/Aachen_Reaction_xpk.png" width="600px"></html>
 
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|'''{{{title|Catalysed reaction by Xpk}}}'''<br />D-Xylulose-5-phosphate + phosphate → Acetyl-phosphate + D-Glyceraldehyde-3-phosphate.
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|'''{{{title|Catalyzed reaction by Xpk}}}'''<br />D-Xylulose-5-phosphate + phosphate → Acetyl-phosphate + D-Glyceraldehyde-3-phosphate.
 
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===Usage and Biology===
 
===Usage and Biology===
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[[Team:Aachen/Aachen_15-08-10_Mdh,_Xpk_f._Wiki_2.png]]
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[[File: Aachen 15-08-10 Mdh, Xpk f. Wiki 2.png]]
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Mdh and Xpk in a pSB1A30 backbone on SDS page after IPTG induction with a RFP expression strain as negative control|subtitle1=all genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction|subtitle2=all genes expressed in BL21 Gold (DE3), 2 ml samples taken from 50 ml main culture in shake flasks after 6h and 21 h after IPTG induction.
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Mdh and Xpk in a pSB1A30 backbone on SDS page after IPTG induction with a RFP expression strain as negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 01:49, 19 September 2015

XPK

D-Xylulose-5-phosphate-phosphoketolase (Xpk) from Bifidobacterium adolescentis, codon optimized for E. coli, converts Xu5p and Pi to actp and G3P


Catalyzed reaction by Xpk
D-Xylulose-5-phosphate + phosphate → Acetyl-phosphate + D-Glyceraldehyde-3-phosphate.



Usage and Biology

Aachen 15-08-10 Mdh, Xpk f. Wiki 2.png

Mdh and Xpk in a pSB1A30 backbone on SDS page after IPTG induction with a RFP expression strain as negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1408
  • 1000
    COMPATIBLE WITH RFC[1000]