Difference between revisions of "Part:BBa K1585203"
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| − | |'''{{{title| | + | |'''{{{title|Catalyzed reaction by Xpk}}}'''<br />D-Xylulose-5-phosphate + phosphate → Acetyl-phosphate + D-Glyceraldehyde-3-phosphate. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | [[File: Aachen 15-08-10 Mdh, Xpk f. Wiki 2.png]] | ||
| − | + | Mdh and Xpk in a pSB1A30 backbone on SDS page after IPTG induction with a RFP expression strain as negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 01:49, 19 September 2015
XPK
D-Xylulose-5-phosphate-phosphoketolase (Xpk) from Bifidobacterium adolescentis, codon optimized for E. coli, converts Xu5p and Pi to actp and G3P
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| Catalyzed reaction by Xpk D-Xylulose-5-phosphate + phosphate → Acetyl-phosphate + D-Glyceraldehyde-3-phosphate. |
Usage and Biology
Mdh and Xpk in a pSB1A30 backbone on SDS page after IPTG induction with a RFP expression strain as negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1408
- 1000COMPATIBLE WITH RFC[1000]