Difference between revisions of "Part:BBa K1585203"

Latest revision as of 01:49, 19 September 2015

XPK

D-Xylulose-5-phosphate-phosphoketolase (Xpk) from Bifidobacterium adolescentis, codon optimized for E. coli, converts Xu5p and Pi to actp and G3P

Catalyzed reaction by Xpk
D-Xylulose-5-phosphate + phosphate → Acetyl-phosphate + D-Glyceraldehyde-3-phosphate.

Usage and Biology

Mdh and Xpk in a pSB1A30 backbone on SDS page after IPTG induction with a RFP expression strain as negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1408
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1585203 short</partinfo>
-phosphoketolase from Bifidobacterium adolescentis, codon optimized for E.coli, converts Xu5p and pi to actp and g3p
+D-Xylulose-5-phosphate-phosphoketolase (Xpk) from ''Bifidobacterium adolescentis'', codon optimized for ''E. coli'', converts Xu5p and Pi to actp and G3P
+<center>
+<div class="figure" style="float:{{{align|center}}}; margin: 0px 10px 10px 0px; border:{{{border|0px solid #aaa}}};width:{{{width|600px}}};padding:10px 10px 0px 0px;">
+{|
+|<html> <img src="https://static.igem.org/mediawiki/parts/0/05/Aachen_Reaction_xpk.png" width="600px"></html>
+|-
+|'''{{{title|Catalyzed reaction by Xpk}}}'''<br />D-Xylulose-5-phosphate + phosphate → Acetyl-phosphate + D-Glyceraldehyde-3-phosphate.
+|}
+</div>
+</center>
+<html></br></html>
-[[File:Aachen_Reaction_xpk.png]]
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+[[File: Aachen 15-08-10 Mdh, Xpk f. Wiki 2.png]]
+Mdh and Xpk in a pSB1A30 backbone on SDS page after IPTG induction with a RFP expression strain as negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction.
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>