Difference between revisions of "Part:BBa K1602018"
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<h1>Humicola insolens cutinase with N-terminal His-Tag</h1> | <h1>Humicola insolens cutinase with N-terminal His-Tag</h1> | ||
<b>cadA</b> is a gene, coding for a cis-aconitate decarboxylase in Aspergillus terreus. The decarboxylase catalyzes the conversion from cis-aconitate to itaconic acid. The reaction takes place in the cytosol of the host and frees one molecule of carbon dioxide per conversion. | <b>cadA</b> is a gene, coding for a cis-aconitate decarboxylase in Aspergillus terreus. The decarboxylase catalyzes the conversion from cis-aconitate to itaconic acid. The reaction takes place in the cytosol of the host and frees one molecule of carbon dioxide per conversion. | ||
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===<h2>Characteristics</h2>=== | ===<h2>Characteristics</h2>=== | ||
Revision as of 01:47, 19 September 2015
Humicola insolens cutinase with N-terminal His-Tag
cadA is a gene, coding for a cis-aconitate decarboxylase in Aspergillus terreus. The decarboxylase catalyzes the conversion from cis-aconitate to itaconic acid. The reaction takes place in the cytosol of the host and frees one molecule of carbon dioxide per conversion.
Characteristics
| Molecular Weight | 21068.85 |
| Residues | 200 |
| Charge | 5.5 |
| Isoelectric Point | 8.1624 |
| A280 Molar Extinction Coefficients | 12950 (reduced) 13200 (cystine bridges) |
| Improbability of expression in inclusion bodies | 0.891 |
| [Data taken from PEPSTATS] |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]