Difference between revisions of "Part:BBa K1640018"

Revision as of 01:47, 19 September 2015

CHlM

ChlM gene from Chlamydomonas reinhardtii, encodes S-Adenosyl-L-methionine:magnesium protoporphyrin IX methyltransferase, a protein involved in chlorophyll biosynthesis.

Usage and Biology

The autotrophic gene ChlM is involved in the chlorophyll biosynthetic pathway in the formation of chlorophyll a (Meinecke et al. 2010). It encodes the enzyme magnesium protoporphyrin IX methyltransferase (MgPMT), involved in the second step of chlorophyll biosynthesis from protoporphyrin IX (Block et al, 2002). ChlM utilizes ubiquitous methylating agent S-Adenosyl-L-methionine (SAM) as a cofactor in the catalysis of Mg-protoporphyrin IX through methyl group transfer, forming Mg-protoporyphyrin IX monomethyl ester (Gibson et al., 1963).

S-adenosyl-L-methionine + magnesium protoporphyrin IX ⇌ S-adenosyl-L-homocysteine + magnesium protoporphyrin IX 13-methyl ester

This part allows E. coli to express the Chlamydomonas reinhardtii-derived ChlM gene involved in the chlorophyll biosynthesis pathway.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 225
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 ===Usage and Biology===
-The autotrophic gene ChlM is involved in the chlorophyll biosynthetic pathway in the formation of chlorophyll a (Meinecke et al. 2010). It encodes the enzyme S-Adenosyl-L-methionine:magnesium protoporphyrin IX methyltransferase (SAM:MgPMT), involved in the second step of chlorophyll biosynthesis from protoporphyrin IX (Block et al, 2002). This enzyme is accumulated through light activation, further activating genes aiding in the synthesis of chlorophyll a in the absence of light (Meinecke et al. 2010).
+The autotrophic gene ChlM is involved in the chlorophyll biosynthetic pathway in the formation of chlorophyll a (Meinecke et al. 2010). It encodes the enzyme magnesium protoporphyrin IX methyltransferase (MgPMT), involved in the second step of chlorophyll biosynthesis from protoporphyrin IX (Block et al, 2002). ChlM utilizes ubiquitous methylating agent S-Adenosyl-L-methionine (SAM) as a cofactor in the catalysis of Mg-protoporphyrin IX through methyl group transfer, forming Mg-protoporyphyrin IX monomethyl ester (Gibson et al., 1963).
-The ubiquitous methylating agent SAM catalyses the conversion of Mg-protoporphyrin IX through methyl group transfer, forming Mg-protoporyphyrin IX monomethyl ester (Gibson et al., 1963).