Difference between revisions of "Part:BBa K1602031"
(One intermediate revision by the same user not shown) | |||
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1602031 short</partinfo> | <partinfo>BBa_K1602031 short</partinfo> | ||
− | + | <html> | |
− | + | <head> | |
+ | <style> | ||
+ | table, td, th { border: 0px solid black; } | ||
+ | </style> | ||
+ | </head> | ||
+ | <body> | ||
+ | |||
+ | <table> | ||
+ | <colgroup width=50% span="2"> | ||
+ | <tr> | ||
+ | <td> | ||
+ | This part enables the production of the whole protein scaffold containing the GBD,PDZ and SH3 domains. This scaffold was used by the iGEM Team TU Darmstadt 2015 to introduce a <i>in vitro</i> immobilized enzyme system. The sequence coding for a His-tag is fused to the C-terminus, improving the purification yield. Fusing the Si4-tag(<a href:"https://parts.igem.org/Part:BBa_K1028000">BBa_K1028000</a>) to the 3' end of the sequence, enables the immobilization of the multi-complex enzyme to a silica surface. | ||
+ | </td> | ||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <!-- BLANK --> | ||
+ | </td> | ||
+ | |||
+ | </tr> | ||
+ | </colgroup> | ||
+ | </table> | ||
+ | </body> | ||
+ | </html> | ||
Line 11: | Line 34: | ||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
− | <partinfo> | + | <partinfo>BBa_K1602030 SequenceAndFeatures</partinfo> |
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
− | <partinfo> | + | <partinfo>BBa_K1602030 parameters</partinfo> |
<!-- --> | <!-- --> |
Latest revision as of 01:36, 19 September 2015
HisTag-Scaffold_Si4-Tag fusion protein with RBS BBa_B0034
This part enables the production of the whole protein scaffold containing the GBD,PDZ and SH3 domains. This scaffold was used by the iGEM Team TU Darmstadt 2015 to introduce a in vitro immobilized enzyme system. The sequence coding for a His-tag is fused to the C-terminus, improving the purification yield. Fusing the Si4-tag(BBa_K1028000) to the 3' end of the sequence, enables the immobilization of the multi-complex enzyme to a silica surface. |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 22
Illegal BamHI site found at 877 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 94
Illegal AgeI site found at 217 - 1000COMPATIBLE WITH RFC[1000]