Difference between revisions of "Part:BBa K1820021"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1820021 short</partinfo>
 
<partinfo>BBa_K1820021 short</partinfo>
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<p>A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31 with the B0034 ribosomal binding site (RBS) (BBa_K1820001), a red fluorescent protein from 2013 team Uppsala, previously termed mCherry, that was codon optimized for ''Lactobacillus reuteri'' (BBa_K1033250), and a transcriptional terminator.</p>  
+
<p>A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31 with the B0034 ribosomal binding site (RBS) (BBa_K1820001), a red fluorescent protein from the 2013 Uppsala team, previously termed mCherry, that was codon optimized for ''Lactobacillus reuteri'' (BBa_K1033250), and a transcriptional terminator.</p>  
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 01:35, 19 September 2015

P335-Φ31 inducible middle promoter + RBS + mCherry + term

A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31 with the B0034 ribosomal binding site (RBS) (BBa_K1820001), a red fluorescent protein from the 2013 Uppsala team, previously termed mCherry, that was codon optimized for Lactobacillus reuteri (BBa_K1033250), and a transcriptional terminator.

Usage and Biology

Transcription from this promoter is induced after phage infection of Lactococcus lactis, resulting in expression of mCherry (previously shown to have fluorescent activity in L. lactis). Allowing for fluorescent detection of Φ31 phage infection in L. lactis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 42