Difference between revisions of "Part:BBa K1820021"
Saragertsch (Talk | contribs) |
Saragertsch (Talk | contribs) |
||
Line 1: | Line 1: | ||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1820021 short</partinfo> | <partinfo>BBa_K1820021 short</partinfo> | ||
− | <p>A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31 with the | + | <p>A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31 with the B0034 ribosomal binding site (RBS) (BBa_K1820001), a red fluorescent protein from 2013 team Uppsala, previously termed mCherry, that was codon optimized for ''Lactobacillus reuteri'' (BBa_K1033250), and a transcriptional terminator.</p> |
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 01:31, 19 September 2015
P335-Φ31 inducible middle promoter + RBS + mCherry + term
A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31 with the B0034 ribosomal binding site (RBS) (BBa_K1820001), a red fluorescent protein from 2013 team Uppsala, previously termed mCherry, that was codon optimized for Lactobacillus reuteri (BBa_K1033250), and a transcriptional terminator.
Usage and Biology
Transcription from this promoter is induced after phage infection of Lactococcus lactis, resulting in expression of mCherry (previously shown to have fluorescent activity in L. lactis). Allowing for fluorescent detection of Φ31 phage infection in L. lactis.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 42