Difference between revisions of "Part:BBa K1807003"

 
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<partinfo>BBa_K1807003 short</partinfo>
 
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This part codes for the Polyphosphate kinase enzyme.
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This part is a protein generator that codes for the Polyphosphate Kinase (PPK) enzyme from Candidatus ''Accumulibacter phosphatis'' BA-91 strain.  This enzyme is responsible for the formation of long chain polyphosphate molecules (up to a thousand orthophosphate residues long) - see illustration below.
  
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===Usage and Biology===
 
===Usage and Biology===
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<img src="https://static.igem.org/mediawiki/2015/d/d4/PPK_activity_York.jpeg" style="width:65%;height:auto">
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The known Candidatus ''Accumulibacter phosphatis'' strains are considered Polyphosphate-Accumulating Organisms (PAOs). They were first discovered in Activated Sludge performing Enhanced Biological Phosphorus Removal in wastewater treatment facilities. Those organisms are capable of accumulating more than two times higher percentage of their dry weight in phosphorus than their non-PAO counterparts. Unfortunately, ''Accumulibacter'' has so far proven unculturable which makes it difficult to examine its genetics and biochemistry. However, metagenomic sequences from enriched cultures have been obtained in recent years and this has enabled the production of phylogenetic analysis and mostly-complete genomic sequence assemblies.
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iGEM York 2015 decided to tap into the PAOs potential and systematically test ''Accumulibacter'' phosphate-uptake and storage related enzymes. Rather than attempt to clone the genes from Activated sludge, iGEM York 2015 designed chemically synthesized DNA based on the metagenomic sequence samples available.
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Latest revision as of 01:02, 19 September 2015

Accummulibacter phosphatis BA-91str. Polyphosphate Kinase gene

This part is a protein generator that codes for the Polyphosphate Kinase (PPK) enzyme from Candidatus Accumulibacter phosphatis BA-91 strain. This enzyme is responsible for the formation of long chain polyphosphate molecules (up to a thousand orthophosphate residues long) - see illustration below.

Usage and Biology

The known Candidatus Accumulibacter phosphatis strains are considered Polyphosphate-Accumulating Organisms (PAOs). They were first discovered in Activated Sludge performing Enhanced Biological Phosphorus Removal in wastewater treatment facilities. Those organisms are capable of accumulating more than two times higher percentage of their dry weight in phosphorus than their non-PAO counterparts. Unfortunately, Accumulibacter has so far proven unculturable which makes it difficult to examine its genetics and biochemistry. However, metagenomic sequences from enriched cultures have been obtained in recent years and this has enabled the production of phylogenetic analysis and mostly-complete genomic sequence assemblies. iGEM York 2015 decided to tap into the PAOs potential and systematically test Accumulibacter phosphate-uptake and storage related enzymes. Rather than attempt to clone the genes from Activated sludge, iGEM York 2015 designed chemically synthesized DNA based on the metagenomic sequence samples available.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 429
    Illegal BamHI site found at 559
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 373