Difference between revisions of "Part:BBa B0011:Design"
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In the naturally-occuring sequence there is a mismatch in the stem of the      stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be      found using some inverted repeat detectors like PALINDROME because it is too      short and contains a mismatch. This one was found with the help of Tom Knight.      It lies between two coding regions that point towards eachother.
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In the naturally-occuring sequence there is a mismatch in the stem of the      stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be      found using some inverted repeat detectors like PALINDROME because it is too      short and contains a mismatch. This one was found with the help of Tom Knight.      It lies between two coding regions that point towards each other.
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===Source===
 
===Source===

Revision as of 15:12, 5 April 2007


LuxICDABEG (+/-)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards each other.

Source

Derived from luxICDABEG operon terminator of Vibrio fischeri [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Search&db=nucleotide&term=AF170104 [Genbank AF170104] ].

References