Difference between revisions of "Part:BBa K1813029"
 
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<partinfo>BBa_K1813029 short</partinfo>
 
<partinfo>BBa_K1813029 short</partinfo>
  
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nicX
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<i>nicX</i> Gen Bank accession number Q88FY1.1.
  
 
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<partinfo>BBa_K1813029 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1813029 SequenceAndFeatures</partinfo>
  
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<html>
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<h2>Background of <i>nicX</i></h2>
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===Functional Parameters===
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<h4>Part Description</h4>
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<partinfo>BBa_K1813029 parameters</partinfo>
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<p>Codon optimized coding sequence of <i>nicX</i>, an enzyme from the <i>Pseudmonas putida</i> bacterium KT2240, for E.Coli. This composite part was used to make our part <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1813030">BBa_K1813030</a> </html> and
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<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1813031">BBa_K1813031</a> </html>.
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<h2>Background for <i>nicX</i></h2>
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<h4>Part Description:</h4>
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<p>description of part</p>
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<h4>Part Origin</h4>
 
<h4>Part Origin</h4>
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<p>where part originated and <i>Genus species</i> more words if needed</p>
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<p>Originally cloned and characterized by Jimenez et al. from <i>Pseudmonas putida</i> KT2240 [1].</p>
  
 
<h4>Part Function</h4>
 
<h4>Part Function</h4>
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<p>part functionstuff </p>
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<p>The enzyme coded for by <i>nicX</i> is a 2,5 DHP dioxygenase that converts 2,5 Dihydroxypyridine (2,5 DHP) to N-Formylmaleamic Acid (NFM).</p>
  
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<h4>Design and Acquisition</h4>
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<h4>References</h4>
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<p>design and acquisition</p>
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[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105
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<h4>Experience</h4>
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<p>design and acquisition </p>
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Latest revision as of 00:55, 19 September 2015

nicX

nicX Gen Bank accession number Q88FY1.1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 332
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 481
    Illegal AgeI site found at 709
    Illegal AgeI site found at 907
  • 1000
    COMPATIBLE WITH RFC[1000]

Background of nicX

Part Description

Codon optimized coding sequence of nicX, an enzyme from the Pseudmonas putida bacterium KT2240, for E.Coli. This composite part was used to make our part BBa_K1813030 and BBa_K1813031 .

Part Origin

Originally cloned and characterized by Jimenez et al. from Pseudmonas putida KT2240 [1].

Part Function

The enzyme coded for by nicX is a 2,5 DHP dioxygenase that converts 2,5 Dihydroxypyridine (2,5 DHP) to N-Formylmaleamic Acid (NFM).

References

[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105