Difference between revisions of "Part:BBa K1631012:Experience"
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| − | We succeeded to control growth rate of <i>E coli</i>. | + | We succeeded to control growth rate of <i>E coli JM109</i> (which expresses LacI excessively). |
<p>We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control. | <p>We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control. | ||
[[File:Yamanaka 002.png|600px|thumb|center|alt text]] | [[File:Yamanaka 002.png|600px|thumb|center|alt text]] | ||
Latest revision as of 00:50, 19 September 2015
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UNIQ0067e5e8cdbb1c83-partinfo-00000000-QINU We succeeded to control growth rate of E coli JM109 (which expresses LacI excessively).
We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control.
We also evaluated the growth rate by slope of next graph.
Plac GFP- 0.0213(/min)
Plac GFP+ 0.0205(/min)
Plac Barnase GFP- 0.0222(/min)
Plac Barnase GFP+ 0.0117(/min)
UNIQ0067e5e8cdbb1c83-partinfo-00000001-QINU