Difference between revisions of "Part:BBa K1631012:Experience"

Latest revision as of 00:50, 19 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1631012

User Reviews

UNIQ0e87b029c389eda8-partinfo-00000000-QINU We succeeded to control growth rate of E coli JM109 (which expresses LacI excessively).

We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control.

alt text

We also evaluated the growth rate by slope of next graph.

alt text

Plac GFP- 0.0213(/min)
Plac GFP+ 0.0205(/min)
Plac Barnase GFP- 0.0222(/min)
Plac Barnase GFP+ 0.0117(/min)

UNIQ0e87b029c389eda8-partinfo-00000001-QINU

@@ Line 7: / Line 7: @@
 ===User Reviews===
 <!-- DON'T DELETE --><partinfo>BBa_K1631012 StartReviews</partinfo>
-We succeeded to control growth rate of <i>E coli</i>.
+We succeeded to control growth rate of <i>E coli JM109</i> (which expresses LacI excessively).
 <p>We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control.
 [[File:Yamanaka 002.png|600px|thumb|center|alt text]]
@@ Line 14: / Line 14: @@
 We also evaluated the growth rate by slope of next graph.
 [[File:Yamanaka 001.png|600px|thumb|center|alt text]]
-Plac GFP-          0.0213
+<br>Plac GFP-          0.0213(/min)
-Plac GFP+          0.0205
+<br>Plac GFP+          0.0205(/min)
-Plac Barnase GFP-  0.0222
+<br>Plac Barnase GFP-  0.0222(/min)
-Plac Barnase GFP+  0.0117
+<br>Plac Barnase GFP+  0.0117(/min)