Difference between revisions of "Part:BBa K1813066"
 
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<partinfo>BBa_K1813066 short</partinfo>
 
<partinfo>BBa_K1813066 short</partinfo>
  
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With the <i> A. gambiae </i> NADPH p450 reductase (<html><b> <a href="https://parts.igem.org/Part:BBa_K1813010">BBa_K1813010</a></b></html>) regulated by the inducible LacI promoter and the CYP6M1vQ (<html><b> <a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K1813005</a></b></html>) enzyme under constitutive regulation, this part allows one to simultaneously gauge the effectiveness of the reductase and the CYP p450, localized to the bacterial periplasm, while remaining a viable method of expression control.
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With the <i> A. gambiae </i> NADPH p450 reductase (<html><b> <a href="https://parts.igem.org/Part:BBa_K1813010">BBa_K1813010</a></b></html>) regulated by the inducible LacI promoter and the CYP2D6(<html><b> <a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K1813005</a></b></html>) enzyme under constitutive regulation, this part allows one to simultaneously gauge the effectiveness of the reductase and the CYP P450, localized to the bacterial periplasm, while remaining a viable method of expression control.
  
  

Latest revision as of 00:50, 19 September 2015

LacIR pelB AgCPR ptac pelB CYP2D6

With the A. gambiae NADPH p450 reductase ( BBa_K1813010) regulated by the inducible LacI promoter and the CYP2D6( BBa_K1813005) enzyme under constitutive regulation, this part allows one to simultaneously gauge the effectiveness of the reductase and the CYP P450, localized to the bacterial periplasm, while remaining a viable method of expression control.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
    Illegal BglII site found at 4110
    Illegal BamHI site found at 1953
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1468
    Illegal NgoMIV site found at 3775
    Illegal NgoMIV site found at 4700
    Illegal AgeI site found at 1751
    Illegal AgeI site found at 2933
    Illegal AgeI site found at 3089
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3449