Difference between revisions of "Part:BBa K1689015:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1689015 short</partinfo>
 
<partinfo>BBa_K1689015 short</partinfo>
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===Design Notes===
 
===Design Notes===
−
the GC% is high of F[1,2]
+
There exists a protein linker: GGGGS*2, the GC% of it is high, so you'd better keep in mind when using Gibson Assembly;
 +
There is an EcoRI restriction site in dCas9, we have eliminated it by site mutation, you can use our part directly or you need pay attention to your dCas9 sequence.
  
  
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===Source===
 
===Source===
  
−
E.coli
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<i>E.coli</i>
  
 
===References===
 
===References===

Revision as of 00:40, 19 September 2015

F[1,2]-dCas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1441
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal BamHI site found at 3720
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There exists a protein linker: GGGGS*2, the GC% of it is high, so you'd better keep in mind when using Gibson Assembly; There is an EcoRI restriction site in dCas9, we have eliminated it by site mutation, you can use our part directly or you need pay attention to your dCas9 sequence.


Source

E.coli

References