Difference between revisions of "Part:BBa K1642010:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | PcpcG2(http://2015.igem.org/Team:SJTU-BioX-Shanghai/ | + | PcpcG2(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Promoters) |
HR(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Transport_Module) | HR(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Transport_Module) | ||
− | PcpcG2-HR | + | PcpcG2-HR(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Desalination_Process) |
===Source=== | ===Source=== | ||
Overlap PCR from basic parts Up(BBa_K1642005), PcpcG2(BBa_K1642002), RBS(BBa_K1642004), HR(BBa_K1642001), Terminator(BBa_B0012), PcpcB-Kana(BBa_K1642003), Down(BBa_K1642006). | Overlap PCR from basic parts Up(BBa_K1642005), PcpcG2(BBa_K1642002), RBS(BBa_K1642004), HR(BBa_K1642001), Terminator(BBa_B0012), PcpcB-Kana(BBa_K1642003), Down(BBa_K1642006). |
Latest revision as of 00:37, 19 September 2015
Expression of Halorhodopsin under the control of PcpcG2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3398
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 481
Illegal NgoMIV site found at 966
Illegal NgoMIV site found at 1125
Illegal NgoMIV site found at 1446
Illegal AgeI site found at 1317 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
PcpcG2(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Promoters)
HR(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Transport_Module)
PcpcG2-HR(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Desalination_Process)
Source
Overlap PCR from basic parts Up(BBa_K1642005), PcpcG2(BBa_K1642002), RBS(BBa_K1642004), HR(BBa_K1642001), Terminator(BBa_B0012), PcpcB-Kana(BBa_K1642003), Down(BBa_K1642006).