Difference between revisions of "Part:BBa K1632006"

Latest revision as of 00:33, 19 September 2015

fim switch[default ON](Tokyo_Tech/R0010)

The fim switch is the promoter containing repeated DNA sequence which is inverted by the Fim recombinase. Therefore, we can control the expression of the gene downstream of the fim switch by adding the Fim recombinase.
We designed the fim switch which has a J23119 promoter(BBa_K1632000) . Basically, the design of the fim switch(Tokyo_Tech) is similar to the fim switch(wild-type). The only difference is that we inserted restriction cut enzyme sites to fim switch(Tokyo_Tech). In detail, in fim switch(Tokyo_Tech) the sigma 70 promoter is exchanged to the J23119 promoter and there are two restriction enzyme cut sites inserted each in the front (SalI and BamHI) and in the back (BglII and MluI) of the promoter. Due to the insertion of the restriction enzyme cut sites, fim switch(Tokyo_Tech) has a promoter which can we exchanged with an arbitrary promoter. We actually changed J23119 promoter in the fim switch(Tokyo_Tech) to Lac promoter(BBa_R0010) as shown in the figure below.

Fig. 1. Fim switch(Tokyo_Tech/R0010) design

More information

For more information, see http://2015.igem.org/Team:Tokyo_Tech/Project Our work in Tokyo_Tech 2015 wiki, http://2015.igem.org/Team:Tokyo_Tech/Experiment/ssrA_tag_degradation_assay About ssrA-tag, http://2015.igem.org/Team:Tokyo_Tech/Experiment/Overview_of_fim_inversion_system About ''fim'' inversion system

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 539
Illegal BamHI site found at 333
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <span style="margin-left: 10px;">The <i>fim</i> switch is the promoter containing repeated DNA sequence which is inverted by the Fim recombinase. Therefore, we can control the expression of the gene downstream of the <i>fim</i> switch by adding the Fim recombinase.<br>
-<span style="margin-left: 10px;">We designed the ''fim'' switch which has a J23119 promoter(<partinfo>BBa_K1632000</partinfo>) . Also between the promoter and the inverting site, there are two restriction enzyme sites in each front (SalIand BamHI) and back (BglII and MluI)(Fig. 1. Tokyo Tech ''fim'' switch (Tokyo_Tech/J23119) design). So the promoter can easily be interchanged. This ''fim'' switch is an exmple of that. Except for insertion of restriction enzyme sites, basically, the design of TokyoTech fim switch is similar with wild type fim switch.
+<span style="margin-left: 10px;">We designed the ''fim'' switch which has a J23119 promoter(<partinfo>BBa_K1632000</partinfo>) . Basically, the design of the ''fim'' switch(Tokyo_Tech) is similar to the ''fim'' switch(wild-type). The only difference is that we inserted restriction cut enzyme sites to ''fim'' switch(Tokyo_Tech). In detail, in ''fim'' switch(Tokyo_Tech) the sigma 70 promoter is exchanged to the J23119 promoter and there are two restriction enzyme cut sites inserted each in the front (SalI and BamHI) and in the back (BglII and MluI) of the promoter. Due to the insertion of the restriction enzyme cut sites, ''fim'' switch(Tokyo_Tech) has a promoter which can we exchanged with an arbitrary promoter. We actually changed J23119 promoter in the ''fim'' switch(Tokyo_Tech) to Lac promoter(<partinfo>BBa_R0010</partinfo>) as shown in the figure below.
+[[Image:Tokyo Tech fim switch Tokyo Tech Plac design.png|thumb|center|600px|Fig. 1. ''Fim'' switch(Tokyo_Tech/R0010) design]]<br>
+===More information===
+For more information, see [[http://2015.igem.org/Team:Tokyo_Tech/Project Our work in Tokyo_Tech 2015 wiki]],   [[http://2015.igem.org/Team:Tokyo_Tech/Experiment/ssrA_tag_degradation_assay About ssrA-tag]],   [[http://2015.igem.org/Team:Tokyo_Tech/Experiment/Overview_of_fim_inversion_system About ''fim'' inversion system]]
 <!-- Add more about the biology of this part here