Difference between revisions of "Part:BBa K1813013"
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<partinfo>BBa_K1813013 short</partinfo>
 
<partinfo>BBa_K1813013 short</partinfo>
  
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A construct used to achieve the overexpression of HUMCYPDB1/CYP2D6 ( <html><b><a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K11813005</b></a></html> in a bacterial host.
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A construct used to achieve the overexpression of HUMCYPDB1/CYP2D6 (<html><b><a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K11813005</b></a></html>) in a bacterial host.
 
Structure: ptac:: HUMCYPDB1:: term.
 
Structure: ptac:: HUMCYPDB1:: term.
  

Revision as of 00:30, 19 September 2015

CYP2D6 Expression Cassette

A construct used to achieve the overexpression of HUMCYPDB1/CYP2D6 (BBa_K11813005) in a bacterial host. Structure: ptac:: HUMCYPDB1:: term.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 410
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1000
  • 1000
    COMPATIBLE WITH RFC[1000]



Background

Powered by the constitutive bacterial promoter ptac, this construct allows the indiscriminate production of HUMCYPDB1 by a bacterial organism harboring this construct. This construct was assembled by Michael VanInsberghe, and is codon optimized for E. coli .
It is used in BBa_K1813024 and BBa_K1813032.