Difference between revisions of "Part:BBa K1807004:Design"

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__NOTOC__
 
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<partinfo>BBa_K1807004 short</partinfo>
 
<partinfo>BBa_K1807004 short</partinfo>
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<img src="https://static.igem.org/mediawiki/2015/3/34/BBa_K1807004_York.png" style="width:50%"; "height:auto">
 
<img src="https://static.igem.org/mediawiki/2015/3/34/BBa_K1807004_York.png" style="width:50%"; "height:auto">
 
</body>
 
</body>
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iGEM York 2015 generated this part by designing two overlapping G Block fragments (999bp and 1245bp) that were used in a Gibson Assembly Reaction with BBa_K1807000.
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<html>
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<body>
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<img src="https://static.igem.org/mediawiki/2015/8/87/SK-12_generation_york.png" style="width:70%"; "height:auto">
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===Source===
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Candidatus ''Accumulibacter phosphatis'' SK-12
  
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<p> ===Source=== </p>
 
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<p>Candidatus ''Accumulibacter phosphatis'' SK-12 strain</p>
 
 
===References===
 
===References===

Latest revision as of 00:28, 19 September 2015

Polyphosphate kinase gene from Accumulibacter phosphatis SK-12


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1063
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 128
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 554
    Illegal SapI site found at 714


Design Notes

iGEM York 2015 generated this part by designing two overlapping G Block fragments (999bp and 1245bp) that were used in a Gibson Assembly Reaction with BBa_K1807000.

Source

Candidatus Accumulibacter phosphatis SK-12

References