Difference between revisions of "Part:BBa K1813008"

 
 
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__NOTOC__
 
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<partinfo>BBa_K1813008 short</partinfo>
 
<partinfo>BBa_K1813008 short</partinfo>
  
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nicE
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<i>nicE</i> Gen Bank accession number Q88FY4.1.
  
 
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<partinfo>BBa_K1813008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1813008 SequenceAndFeatures</partinfo>
  
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<html>
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<h2>Background of <i>nicE</i></h2>
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<h4>Part Description</h4>
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<p>Codon optimized coding sequence of <i>nicE</i>, an enzyme from the <i>Pseudmonas putida</i> bacterium KT2240, for E.Coli. This composite part was used to make our part <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1813016">BBa_K1813016</a> </html> and
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<html><a href="https://parts.igem.org/Part:BBa_K1813022">BBa_K1813022</a> </html>.
  
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<h4>Part Origin</h4>
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===Functional Parameters===
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<p>Originally cloned and characterized by Jimenez et al. from <i>Pseudmonas putida</i> KT2240 [1].</p>
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<partinfo>BBa_K1813008 parameters</partinfo>
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<h4>Part Function</h4>
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<p>The enzyme coded for by <i>nicE</i> is a maleate isomerase that converts Maleic Acid to Fumaric Acid.</p>
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<h4>References</h4>
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[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105

Latest revision as of 00:27, 19 September 2015

nicE

nicE Gen Bank accession number Q88FY4.1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 640
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 55

Background of nicE

Part Description

Codon optimized coding sequence of nicE, an enzyme from the Pseudmonas putida bacterium KT2240, for E.Coli. This composite part was used to make our part BBa_K1813016 and BBa_K1813022 .

Part Origin

Originally cloned and characterized by Jimenez et al. from Pseudmonas putida KT2240 [1].

Part Function

The enzyme coded for by nicE is a maleate isomerase that converts Maleic Acid to Fumaric Acid.

References

[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105