Difference between revisions of "Part:BBa K1583001"

 
 
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<partinfo>BBa_K1583001 short</partinfo>
 
<partinfo>BBa_K1583001 short</partinfo>
  
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CsgC is positively involved in the extracellular aggregation of CsgA into amyloid nanowires (curli assembly) in E.coli functioning as a chaperone for CsgA.
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<p>CsgC prevents CsgA proteins from self-assembling inside the cell. CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms of <i>E.coli</i> (Barnhart & Chapman 2010).</p>
  
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<p><i>csgC</i> is used in the biobricks:
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===Usage and Biology===
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<ul><li><a href="https://parts.igem.org/Part:BBa_K1583200"link+_blank">BBa_K1583200</a></li>
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<li><a href="https://parts.igem.org/Part:BBa_K1583201"link+_blank">BBa_K1583201</a></li>
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<li><a href="https://parts.igem.org/Part:BBa_K1583202"link+_blank">BBa_K1583202</a></li>
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<li><a href="https://parts.igem.org/Part:BBa_K1583203"link+_blank">BBa_K1583203</a></li>
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<li><a href="https://parts.igem.org/Part:BBa_K1583204"link+_blank">BBa_K1583204</a></li>
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<li><a href="https://parts.igem.org/Part:BBa_K1583205"link+_blank">BBa_K1583205</a></li></ul></p>
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<p>The first three parts contain CsgC with a low constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23110">BBa_J23110</a>), a medium constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23118">BBa_J23118</a>) and a high consitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a>), respectively.</p>
  
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<p>The other three parts contain both CsgC and CsgB. These induced are by a low constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23110">BBa_J23110</a>), a medium constitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23118">BBa_J23118</a>) and a high consitutive promoter (<a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a>), respectively.</p>
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><h3>Sequence and Features</h3></span>
 
<partinfo>BBa_K1583001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1583001 SequenceAndFeatures</partinfo>
  
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<h3>References</h3>
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<p>Barnhart, M. M., & Chapman, M. R. (2010). Curli Biogenesis and Function, 4945.</p>
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===Functional Parameters===
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<partinfo>BBa_K1583001 parameters</partinfo>
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Latest revision as of 00:24, 19 September 2015

CsgC

CsgC prevents CsgA proteins from self-assembling inside the cell. CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms of E.coli (Barnhart & Chapman 2010).

csgC is used in the biobricks:

The first three parts contain CsgC with a low constitutive promoter (BBa_J23110), a medium constitutive promoter (BBa_J23118) and a high consitutive promoter (BBa_J23100), respectively.

The other three parts contain both CsgC and CsgB. These induced are by a low constitutive promoter (BBa_J23110), a medium constitutive promoter (BBa_J23118) and a high consitutive promoter (BBa_J23100), respectively.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Barnhart, M. M., & Chapman, M. R. (2010). Curli Biogenesis and Function, 4945.