Difference between revisions of "Part:BBa K1813008"
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<partinfo>BBa_K1813008 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1813008 SequenceAndFeatures</partinfo> | ||
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<h2>Background of <i>nicE</i></h2> | <h2>Background of <i>nicE</i></h2> | ||
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<p>Codon optimized coding sequence of <i>nicE</i>, an enzyme from the <i>Pseudmonas putida</i> bacterium KT2240, for E.Coli. This composite part was used to make our part <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1813016">BBa_K1813016</a> </html> and | <p>Codon optimized coding sequence of <i>nicE</i>, an enzyme from the <i>Pseudmonas putida</i> bacterium KT2240, for E.Coli. This composite part was used to make our part <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1813016">BBa_K1813016</a> </html> and | ||
<html><a href="https://parts.igem.org/Part:BBa_K1813022">BBa_K1813022</a> </html>. | <html><a href="https://parts.igem.org/Part:BBa_K1813022">BBa_K1813022</a> </html>. | ||
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<h4>Part Origin:</h4> | <h4>Part Origin:</h4> |
Revision as of 23:57, 18 September 2015
nicE
nicE
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 640
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 55
Background of nicE
Part Description:
Codon optimized coding sequence of nicE, an enzyme from the Pseudmonas putida bacterium KT2240, for E.Coli. This composite part was used to make our part BBa_K1813016 and BBa_K1813022 .
Part Origin:
Originally cloned and characterized by Jimenez et al. from Pseudmonas putida KT2240 [1].
Part Function:
The enzyme coded for by nicE is a maleate isomerase that converts Maleic Acid to Fumaric Acid.
References:
[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105