Difference between revisions of "Part:BBa K1813006"
 
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<html>
 
<html>
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<h2>Background of <i>nicC</i>:</h2>
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<h2>Background of <i>nicC</i></h2>
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<h4>Part Description:</h4>
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<h4>Part Description</h4>
 
<p>Codon optimized coding sequence of <i>nicC</i>, an enzyme from the <i>Pseudmonas putida</i> bacterium KT2240, for E.Coli. This composite part was used to make our part <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1813020">BBa_K1813020</a> </html> and  
 
<p>Codon optimized coding sequence of <i>nicC</i>, an enzyme from the <i>Pseudmonas putida</i> bacterium KT2240, for E.Coli. This composite part was used to make our part <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1813020">BBa_K1813020</a> </html> and  
 
<html><a href="https://parts.igem.org/Part:BBa_K1813014">BBa_K1813014</a> </html>.  
 
<html><a href="https://parts.igem.org/Part:BBa_K1813014">BBa_K1813014</a> </html>.  
  
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<h4>Part Origin:</h4>
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<h4>Part Origin</h4>
 
<p>Originally cloned and characterized by Jimenez et al. from <i>Pseudmonas putida</i> KT2240 [1].</p>
 
<p>Originally cloned and characterized by Jimenez et al. from <i>Pseudmonas putida</i> KT2240 [1].</p>
  
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<h4>Part Function:</h4>
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<h4>Part Function</h4>
 
<p>The enzyme coded for by <i>nicC</i> is a 6-HNA monooxygenase that converts 6-Hydroxynicitinoic acid (6-HNA) to 2,5-dihydroxypyridine (2,5-DHP).</p>
 
<p>The enzyme coded for by <i>nicC</i> is a 6-HNA monooxygenase that converts 6-Hydroxynicitinoic acid (6-HNA) to 2,5-dihydroxypyridine (2,5-DHP).</p>
  
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<h4>References:</h4>
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<h4>References</h4>
 
[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105
 
[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105

Latest revision as of 23:55, 18 September 2015

nicC

nicC Gen Bank accession number Q88FY2.1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 244
  • 1000
    COMPATIBLE WITH RFC[1000]

Background of nicC

Part Description

Codon optimized coding sequence of nicC, an enzyme from the Pseudmonas putida bacterium KT2240, for E.Coli. This composite part was used to make our part BBa_K1813020 and BBa_K1813014 .

Part Origin

Originally cloned and characterized by Jimenez et al. from Pseudmonas putida KT2240 [1].

Part Function

The enzyme coded for by nicC is a 6-HNA monooxygenase that converts 6-Hydroxynicitinoic acid (6-HNA) to 2,5-dihydroxypyridine (2,5-DHP).

References

[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105