Difference between revisions of "Part:BBa K1813007"
 
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<partinfo>BBa_K1813007 short</partinfo>
 
<partinfo>BBa_K1813007 short</partinfo>
  
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nicD
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<i>nicD</i> Gen Bank accession number Q88FY3.1.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1813007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1813007 SequenceAndFeatures</partinfo>
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<html>
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<h2>Background of <i>nicD</i>:</h2>
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<h2>Background of <i>nicD</i></h2>
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<h4>Part Description:</h4>
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<h4>Part Description</h4>
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<p>Codon optimized coding sequence of <i>nicD</i>, an enzyme from the <i>Pseudmonas putida</i> bacterium KT2240, for E.Coli. This composite part was used to make our part <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1813007">BBa_K1813007</a>. </p>
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<p>Codon optimized coding sequence of <i>nicD</i>, an enzyme from the <i>Pseudmonas putida</i> bacterium KT2240, for E.Coli. This composite part was used to make our part <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1813015">BBa_K1813015</a> </html> and
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<html><a href="https://parts.igem.org/Part:BBa_K1813021">BBa_K1813021</a> </html>.
  
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<h4>Part Origin:</h4>
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<h4>Part Origin</h4>
 
<p>Originally cloned and characterized by Jimenez et al. from <i>Pseudmonas putida</i> KT2240 [1].</p>
 
<p>Originally cloned and characterized by Jimenez et al. from <i>Pseudmonas putida</i> KT2240 [1].</p>
  
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<h4>Part Function:</h4>
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<h4>Part Function</h4>
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<p>The enzyme coded for by <i>nicD</i> converts N-FormylMaleamic (NFM) to Maleamic Acid (2,5-DHP).</p>
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<p>The enzyme coded for by <i>nicD</i> is a N-Formylmaleamate Deformylase that converts N-Formylmaleamic (NFM) to Maleamic Acid, also producing formic acid.</p>
  
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<h4>References:</h4>
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<h4>References</h4>
 
[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105
 
[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105

Latest revision as of 23:54, 18 September 2015

nicD

nicD Gen Bank accession number Q88FY3.1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 727
  • 1000
    COMPATIBLE WITH RFC[1000]

Background of nicD

Part Description

Codon optimized coding sequence of nicD, an enzyme from the Pseudmonas putida bacterium KT2240, for E.Coli. This composite part was used to make our part BBa_K1813015 and BBa_K1813021 .

Part Origin

Originally cloned and characterized by Jimenez et al. from Pseudmonas putida KT2240 [1].

Part Function

The enzyme coded for by nicD is a N-Formylmaleamate Deformylase that converts N-Formylmaleamic (NFM) to Maleamic Acid, also producing formic acid.

References

[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105