Difference between revisions of "Part:BBa K1585213"
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<partinfo>BBa_K1585213 short</partinfo>
 
<partinfo>BBa_K1585213 short</partinfo>
  
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This is the translational unit for the D-Xylulose-5-phosphate-phosphoketolase (Xpk). It derives from Bifidobacterium adolescentis and is codon optimized for E. coli.
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This is the translational unit for the D-Xylulose-5-phosphate-phosphoketolase (Xpk). It derives from ''Bifidobacterium adolescentis'' and is codon optimized for ''E. coli''.
  
  
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|<html> <img src="https://static.igem.org/mediawiki/parts/0/05/Aachen_Reaction_xpk.png" width="600px"></html>
 
|<html> <img src="https://static.igem.org/mediawiki/parts/0/05/Aachen_Reaction_xpk.png" width="600px"></html>
 
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|'''{{{title|Catalysed reaction by Xpk}}}'''<br />D-Xylulose-5-phosphate + phosphate → Acetyl-phosphate + D-Glyceraldehyde-3-phosphate.
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|'''{{{title|Catalyzed reaction by Xpk}}}'''<br />D-Xylulose-5-phosphate + phosphate → Acetyl-phosphate + D-Glyceraldehyde-3-phosphate.
 
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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[[File: Aachen 15-08-10 Mdh, Xpk f. Wiki 2.png]]
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Mdh and Xpk in a pSB1A30 backbone on SDS page after IPTG induction with a RFP expression strain as negative control|subtitle1=all genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction|subtitle2=all genes expressed in BL21 Gold (DE3), 2 ml samples taken from 50 ml main culture in shake flasks after 6h and 21 h after IPTG induction.
  
 
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Revision as of 23:51, 18 September 2015

B0034.XPK

This is the translational unit for the D-Xylulose-5-phosphate-phosphoketolase (Xpk). It derives from Bifidobacterium adolescentis and is codon optimized for E. coli.


Catalyzed reaction by Xpk
D-Xylulose-5-phosphate + phosphate → Acetyl-phosphate + D-Glyceraldehyde-3-phosphate.



Usage and Biology

Aachen 15-08-10 Mdh, Xpk f. Wiki 2.png

Mdh and Xpk in a pSB1A30 backbone on SDS page after IPTG induction with a RFP expression strain as negative control|subtitle1=all genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction|subtitle2=all genes expressed in BL21 Gold (DE3), 2 ml samples taken from 50 ml main culture in shake flasks after 6h and 21 h after IPTG induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1426
  • 1000
    COMPATIBLE WITH RFC[1000]