Difference between revisions of "Part:BBa K1582007"

 
 
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mHGFI is a small protein produced by filamentous fungi, which use serine to replace all of HGFI's conserved eight-cysteine residues.Hydrophobins are amphipathic, which means they have the hydrophobic part and hydrophilic part at the same time. mHGFI is a kind of hydrophobins and it has magic properties. We combine GFP with mHGFI by a linker and make them become a fusion protein. This fusion protein could be used in aqueous two-phase systems to make itself seperated frome mixture. Meanwile, hydrophobin has a property that it can generate a membrane on the surface of solid automatically, which is called self-assembly. We can get a fluorescent protein' membrane by it.
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===Usage===
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Admittedly the separation of proteins is difficult in biological experiments. More often than not, we use chromatography, but it is expensive. Extraction turns to be cheap, however, its low separation rate is really an obstacle. <br>
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Here comes the Janus, a kind of amphipathic protein, which is made into a fusion protein with the target and carry it to leave the bulk protein phase. We could use aqueous two-phase systems to make them separated from mixture, according to the property that Janus will direct to the phase of detergent in the system of detergent/polymer. GFP-sJanus fusion protein could be used to verify this purification system. <br>
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===Biology===
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inJanus are small secreted fungal proteins which can be found in filamentous fungi, which play a role in a broad range of processes in the growth and development of filamentous fungi. They shows rod-like structure, can be expressed in eukaryotic cells, and are involved in transferring themselves to detergent phase spontaneously. We did some mutations to it, in order to make it expressed in E.coli, and we call it inJanus-m.
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===Reference===
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[1]Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9751 NN Haren, The Netherlands. Hydrophobins: multipurpose proteins.Annu Rev Microbiol. 2001;55:625-46.

Latest revision as of 23:30, 18 September 2015

GFP+inJanus-m Fusion Protein


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 910
    Illegal BsaI.rc site found at 644


Usage

Admittedly the separation of proteins is difficult in biological experiments. More often than not, we use chromatography, but it is expensive. Extraction turns to be cheap, however, its low separation rate is really an obstacle.
Here comes the Janus, a kind of amphipathic protein, which is made into a fusion protein with the target and carry it to leave the bulk protein phase. We could use aqueous two-phase systems to make them separated from mixture, according to the property that Janus will direct to the phase of detergent in the system of detergent/polymer. GFP-sJanus fusion protein could be used to verify this purification system.

Biology

inJanus are small secreted fungal proteins which can be found in filamentous fungi, which play a role in a broad range of processes in the growth and development of filamentous fungi. They shows rod-like structure, can be expressed in eukaryotic cells, and are involved in transferring themselves to detergent phase spontaneously. We did some mutations to it, in order to make it expressed in E.coli, and we call it inJanus-m.

Reference

[1]Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9751 NN Haren, The Netherlands. Hydrophobins: multipurpose proteins.Annu Rev Microbiol. 2001;55:625-46.