Difference between revisions of "Part:BBa K1585203"

Revision as of 23:19, 18 September 2015

XPK

D-Xylulose-5-phosphate-phosphoketolase (Xpk) from Bifidobacterium adolescentis, codon optimized for E. coli, converts Xu5p and Pi to actp and G3P

Catalysed reaction by Xpk
D-Xylulose-5-phosphate + phosphate → Acetyl-phosphate + D-Glyceraldehyde-3-phosphate.

Usage and Biology

Mdh, Hps and Phi expressed in a pSB1A30 backbone on SDS-PAGE after IPTG induction with a RFP expression strain as a negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1408
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1585203 short</partinfo>
-D-Xylulose-5-phosphate-phosphoketolase (Xpk) from Bifidobacterium adolescentis, codon optimized for E.coli, converts Xu5p and Pi to actp and G3P
+D-Xylulose-5-phosphate-phosphoketolase (Xpk) from ''Bifidobacterium adolescentis'', codon optimized for ''E. coli'', converts Xu5p and Pi to actp and G3P
@@ Line 17: / Line 17: @@
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+[[File:Aachen 15-08-10 Mdh, Hps, Phi f. Wiki 2.png]]
+Mdh, Hps and Phi expressed in a pSB1A30 backbone on SDS-PAGE after IPTG induction with a RFP expression strain as a negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction.
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