Difference between revisions of "Part:BBa K1585202"
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| − | 6-Phospho-3-hexuloisomerase from Bacillus methanolicus MGA3, codon optimized for ''E. coli'', converts D-Arabino-hex-3-ulose-6-phosphate to D-Fructose-6-phosphate | + | 6-Phospho-3-hexuloisomerase from ''Bacillus methanolicus'' MGA3, codon optimized for ''E. coli'', converts D-Arabino-hex-3-ulose-6-phosphate to D-Fructose-6-phosphate |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 23:18, 18 September 2015
PHI
6-Phospho-3-hexuloisomerase from Bacillus methanolicus MGA3, codon optimized for E. coli, converts D-Arabino-hex-3-ulose-6-phosphate to D-Fructose-6-phosphate
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| Catalyzed reaction by Phi D-arabino-hex-3-ulose 6-phosphate → D-fructose 6-phosphate. |
Usage and Biology
Mdh, Hps and Phi expressed in a pSB1A30 backbone on SDS-PAGE after IPTG induction with a RFP expression strain as a negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]