Difference between revisions of "Part:BBa K1799007:Design"
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===Design Notes=== | ===Design Notes=== | ||
This version of the open reading frame has been optimized for modularization via silent mutations of the LsrC RBS and ATG, as well as any segments of code sharing more than 83% homology with the Shine-Dalgarno sequence. It is otherwise identical to the wild type LsrA gene (https://parts.igem.org/Part:BBa_K1799003). | This version of the open reading frame has been optimized for modularization via silent mutations of the LsrC RBS and ATG, as well as any segments of code sharing more than 83% homology with the Shine-Dalgarno sequence. It is otherwise identical to the wild type LsrA gene (https://parts.igem.org/Part:BBa_K1799003). | ||
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===Source=== | ===Source=== | ||
Latest revision as of 23:14, 18 September 2015
LsrA (modularization optimized)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This version of the open reading frame has been optimized for modularization via silent mutations of the LsrC RBS and ATG, as well as any segments of code sharing more than 83% homology with the Shine-Dalgarno sequence. It is otherwise identical to the wild type LsrA gene (https://parts.igem.org/Part:BBa_K1799003).
Source
This part was synthesized, and is based on E. coli genomic data retrieved via EcoGene: http://www.ecogene.org/gene/EG13806