Difference between revisions of "Part:BBa K1585201"
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<partinfo>BBa_K1585201 short</partinfo> | <partinfo>BBa_K1585201 short</partinfo> | ||
| − | 3-hexulose-6-phosphate synthase from Bacillus methanolicus MGA3, codon optimized for E.coli | + | 3-hexulose-6-phosphate synthase from Bacillus methanolicus MGA3, codon optimized for ''E. coli'' |
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|<html> <img src="https://static.igem.org/mediawiki/parts/b/b8/Aachen_Reaction_hps.png" width="600px"></html> | |<html> <img src="https://static.igem.org/mediawiki/parts/b/b8/Aachen_Reaction_hps.png" width="600px"></html> | ||
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| − | |'''{{{title| | + | |'''{{{title|Catalyzed reaction by Hps}}}'''<br />Formaldehyde + Ribulose-5-phosphat → D-arabino-hex-3-ulose 6-phosphate. |
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Latest revision as of 23:07, 18 September 2015
HPS
3-hexulose-6-phosphate synthase from Bacillus methanolicus MGA3, codon optimized for E. coli
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| Catalyzed reaction by Hps Formaldehyde + Ribulose-5-phosphat → D-arabino-hex-3-ulose 6-phosphate. |
Usage and Biology
Mdh, Hps and Phi expressed in a pSB1A30 backbone on SDS-PAGE after IPTG induction with a RFP expression strain as a negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]