Difference between revisions of "Part:BBa K1627002"

 
 
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This part can be used in a &#8710;guaB strain of E. coli to measure the amount of 7-methylxanthine in a solution. By growing the &#8710;guaB strain with this device in a minimal media with different known concentrations of 7-methylxanthine, a growth curve can be made that correlates culture growth with concentration of 7-methylxanthine. Then if the strain is grown in a minimal media with an unknown concentration of 7-methylxanthine, the growth can be compared to the growth curve to calculate the initial concentration of 7-methylxanthine in the media.
 
This part can be used in a &#8710;guaB strain of E. coli to measure the amount of 7-methylxanthine in a solution. By growing the &#8710;guaB strain with this device in a minimal media with different known concentrations of 7-methylxanthine, a growth curve can be made that correlates culture growth with concentration of 7-methylxanthine. Then if the strain is grown in a minimal media with an unknown concentration of 7-methylxanthine, the growth can be compared to the growth curve to calculate the initial concentration of 7-methylxanthine in the media.
  
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See the [http://2015.igem.org/Team:Austin_UTexas/Project/Caffeine 2015 Austin UTexas wiki].
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===Usage and Biology===
 
===Usage and Biology===
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[[Image:2015_Austin_UTexas_pDCAFC_Growth_Curve.png|500px|thumb|left]]
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This device was grown in the ∆guaB strain in minimal media cultures with different concentrations of 7-methylxanthine. As the concentration of 7-methylxanthine increases (up to the point of saturation, after which the strain won't grow further), the culture density increases linearly. By comparing growth from unknown concentrations of 7-methylxanthine to the standard curve, the concentration of 7-methylxanthine can be measured.
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Latest revision as of 22:58, 18 September 2015

Device to demethylate 7-methylxanthine

This part contains genes that allow for the demethylation of 7-methylxanthine. The part contains a synthetic operon consisting of ndmC, ndmD from Pseudomonas putida CBB5 and gst9 from Janthinobacterium sp. Marseille under the control of a constitutive promoter, J23110.

This part can be used in a ∆guaB strain of E. coli to measure the amount of 7-methylxanthine in a solution. By growing the ∆guaB strain with this device in a minimal media with different known concentrations of 7-methylxanthine, a growth curve can be made that correlates culture growth with concentration of 7-methylxanthine. Then if the strain is grown in a minimal media with an unknown concentration of 7-methylxanthine, the growth can be compared to the growth curve to calculate the initial concentration of 7-methylxanthine in the media.

See the [http://2015.igem.org/Team:Austin_UTexas/Project/Caffeine 2015 Austin UTexas wiki].

Usage and Biology

2015 Austin UTexas pDCAFC Growth Curve.png

This device was grown in the ∆guaB strain in minimal media cultures with different concentrations of 7-methylxanthine. As the concentration of 7-methylxanthine increases (up to the point of saturation, after which the strain won't grow further), the culture density increases linearly. By comparing growth from unknown concentrations of 7-methylxanthine to the standard curve, the concentration of 7-methylxanthine can be measured.










Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 498
    Illegal BglII site found at 994
    Illegal BglII site found at 1465
    Illegal XhoI site found at 2606
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3304
    Illegal AgeI site found at 1133
  • 1000
    COMPATIBLE WITH RFC[1000]